Annette Dirac studied Biology at the University of Aarhus, Denmark and also obtained her PhD from there with a thesis regarding aspects of the molecular biology of the Human Immunodeficiency Virus (HIV). Following this she worked as a researcher at the Netherlands Cancer Institute, focusing on genetic screening to identify new genes involved in cancer development, and managing her group’s participation in a European Network of Excellence from 2007 - 2011. Annette joined a grant advising agency for a year and a half, gaining vast experience in EU grant applications. She also supported a range of Dutch grant applications, and was involved in the project management of multi-partner EU projects. Annette has been working as an advisor research funding at the Technology Transfer Office since July 2013.
Kuiken HJ, Egan DA, Laman H, Bernards R, Beijersbergen RL, Dirac AM. Identification of F-box only protein 7 as a negative regulator of NF-kappaB signaling. J cell Mol Med, 2012 Sep;16(9):2140-9.
Eichhorn PJA, Rodón L, Gonzàlez-Juncà A, Dirac AM, Gili M, Martínez-Sáez E, Aura C, Barba I, Prat A, Cuartas I, Jimenez J, García-Dorado D, Bernards R, Baselga J, Seoane J. USP15 stabilizes the TGFb receptor I and is responsible for the hyperactivation of the TGFb signal in glioblastoma. Nat Med. 2012 Feb 19;18(3):429-35.
Faesen AC, Dirac AM, Shanmugham A, Ovaa H, Perrakis A, Sixma TK. Mechanism of USP7/HAUSP activation by its C-terminal ubiquitin-like domain (HUBL) and allosteric regulation by GMP-synthetase. Mol. Cell, 2011 Oct 7;44(1):147-59.
Moretti J, Chastagner P, Gastaldello S, Heuss S, Dirac AM, Bernards R, Masucci M, Israel A, and Brou C. The translation initiation factor 3f (eIF3f) exhibits a deubiquitinase activity regulating Notch activation. PLoS Biol. 2010 Nov 23;8(11):e1000545.
Dirac AM, Bernards R. The deubiquitinating enzyme USP26 is a regulator of Androgen Receptor signaling. Mol Cancer Res. 2010 Jun 8(6):844-54.
Naarding MA, Dirac AM, Ludwig IS, Speijer D, Lindquist S, Vestman EL, Stax MJ, Geijtenbeek TB, Pollakis G, Hernell O, Paxton WA. Bile salt-stimulated lipase from human milk binds DC-SIGN and inhibits human immunodeficiency virus type 1 transfer to CD4+ T cells. Antimicrob Agents Chemother. 2006 Oct;50(10):3367-74.
Oosterkamp HM, Neering H, Nijman SM, Dirac AM, Mooi WJ, Bernards R and Brummelkamp TR. An Evaluation of the Efficacy of Topical Application of Salicylic Acid for the Treatment of Familial Cylindromatosis. Br.J.Derm. 2006, jul;155(1):182-5.
Nijman SM*, Huang T*, Dirac AM*, Brummelkamp TR, Kerkhoven RM, D’Andrea AD, Bernards R. The de-ubiquitinating enzyme USP1 regulates the Fanconi Anemia pathway. Mol Cell. 2005 Feb 4;17(3):331-9.
Brummelkamp TR*, Nijman SM*, Dirac AM*, Bernards R. Loss of the cylindromatosis tumour suppressor inhibits apoptosis by activating NF-kappaB. Nature. 2003 Aug 14;424(6950):797-801.
Dirac AM, Bernards R. Reversal of senescence in mouse fibroblasts through lentiviral suppression of p53. J Biol Chem. 2003 Apr 4;278(14):11731-4.
Dirac AM, Huthoff H, Kjems J, Berkhout B. Requirements for RNA-heterodimerization of the HIV-1 and HIV-2 genomes. J Gen Virol. 2002 Oct;83(Pt 10):2533-42.
Dirac AM, Huthoff H, Kjems J, Berkhout B. Regulated HIV-2 RNA Dimerization by means of Alternative RNA Conformations. Nucleic Acids Res. 2002 Jun 15;30(12):2647-55..
Dirac AM, Huthoff H, Kjems J, Berkhout B. The Dimer Initiation Site Hairpin Mediates Dimerization of the Human Immunodeficiency Virus Type 2 RNA Genome. Jour. Biol. Chem. 2001, 276: 32345-52.
Dirac AM & Kjems J. Mapping DNA binding sites of HIV 1 integrase by protein footprinting. Eur. J. Biochem. 2001, 268: 743-51.
Otero G, Fellows J, Li Y, de Bizemont T, Dirac AM, Gustafsson CM, Erdjument-Bromage H, Tempst P, Svejstrup JQ. Elongator, a multisubunit component of a novel RNA polymerase II holoenzyme for transcriptional elongation. Mol. Cell 1999 Jan;3(1):109-18.
* These authors contributed equally
Dirac AM & Bernards R. Functional identification of critical targets of the Ubiquitin-Proteasome pathway. ASCO 2007 Educational book: 699-703.
Dirac AM, Nijman SM, Brummelkamp TR , Bernards R. Functional annotation of de-ubiquitinating enzymes using RNA interference. Methods in Enzymology, 2005;398:554-67.
Nijman SM, Luna-Vargas MP, Velds A, Brummelkamp TR, Dirac AM, Sixma TK, Bernards R. A genomic and functional inventory of deubiquitinating enzymes. Cell. 2005 Dec 2;123(5):773-86.